Dear All,
I seem to have lost the plasmid DNA of one of my clones for protein expression.However, I do have a glycerol stock of BL21(DE3) cells transformed with the clone. Would it possible to a inoculate a culture with the glycerol stock and miniprep fresh plasmid DNA from the BL21(DE3) culture. As a consequence of the BL21(DE3) host should I expect any modifications on the DNA. I imagine yield will not be as good as that from an amplification strain such as dh5alpha but is this the only drawback.
It will work fine and you should get a pretty normal plasmid yield. Just be sure that you don't have IPTG in the medium so you don't induce protein expression.
It will work fine and you should get a pretty normal plasmid yield. Just be sure that you don't have IPTG in the medium so you don't induce protein expression.
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