Is it possible to perform GeNorm analysis with only 6 cDNA samples? (10 recommended in all publications) Would the software even work with less data?

How to compare two groups with only two measurements?

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

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What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

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What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

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Do we need to write protocol for a second study of a systematic review that has been registered and published?

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How to increase a model accuracy ?

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How do I increase the yield of my PCR product?

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Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

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Sample dilution for quantitative analysis using ELISA?

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Mikhail V Matz

Hi Romana - If you do R, why don't you try my package MCMC.qpcr (there is a detailed tutorial on my RG profile page...). It will work with 6 samples, although the power might be limited (you can compensate by analyzing more genes, though)

Romana Šašková

Hi Mikhail

Thank you for your suggestion, but I do not R :-( but I will download your package and maybe I could learn something from it.

Matt J Neville

HI,

It will work with as many samples you give it, however, as with everything the fewer the samples the less you can say for certain your samples are representative of a larger set. If that is all you have though and those are the samples you will be analysing then Genorm will identify which are best reference genes to use

Mario Hirata

I agree with Matt

Hi Matt

Thanks a lot for assuring me. I was quite nervous about that I'm just before my first Genorm analysis. Hope everything will go smoothly.