the electron shared from keto oxygen of phosphate with other nucleotide base phosphate?
ligation without using ligase
The answer appears to be yes, if the 5' and 3' ends are chemically modified. See this paper for an example:
"High sequence fidelity in a non-enzymatic DNA autoligation reaction"
http://nar.oxfordjournals.org/content/27/3/875.full
Dear Sir, Did you ever tried such enzymatic reaction, If Yes can this happens in-vivo? I am looking for the explanation.
I have ligated DNA using DNA ligase enzyme. I have never tried non-enzymatic ligation.
Dear Sir, Is it possible to check these non-enzymatic ligation by some technique? If yes can we discuss the possibility? How do we quantify them in in-vivo conditions?
In vitro, DNA ligation can be tested by a variety of methods, the most common being agarose gel electrophoresis in the presence of urea. To test it in vivo, you would have to take a sample, extract the DNA, and perform the test. To recognize the specific piece of DNA, you would have to label it in some way, such as with radioactivity, biotin, or a fluorescent dye.
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