Protocol
Hi Mohamed,
try Clotilde Théry protocol.
You can isolate exosome from cell culture. You need to use exosome free medium to culture your cells and for isolation there is 2 possibilities: ultracentifugation (Clotilde Thery) or exosome purification kit.
Thank you, but i need to isolate the exosomes which are still inside the cell
i'm not sur that it is possible because you have to disrupt plasmic membrane.... exosomes need to be secreted if you want to isolate them.
I think this is a hard process. Unless they have a specific marker to identify, you will not be able to collect them.
Source to get Impact factor of journals
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