The heart contains a reservoir of stem and progenitor cells in niches, which may proliferate and protect damaged heart. I want to know to which extent these cells get activated in an infarcted heart. Nowadays extensive research work is going on in isolating cardiac stem cells from the heart and delivering them on to an infarcted heart. I want to know if cardiac stem cells can be isolated from an infarcted heart? If yes, what will be the percentage difference in availability of CSCs from a normal and from infarcted heart biopsies?
Ischaemia/reperfusion induced cardiac stem cell homing to the injured myocardium by stimulating stem cell factor expression via NF-kappaB pathway.
Guo J, Jie W, Kuang D, Ni J, Chen D, Ao Q, Wang G.
Dear Reddy,
We isolate cardiac stem cells from infarcted human hearts based on their ALDH-expression. An average of 0.9% CASCs can be isolated from these hearts. These cells can easily be expanded and differentiate down the cardiomyogenic lineage.So it is possible to isolate CSCs from infarcted hearts. We isolate our cells from right atrial appendages and do not see a major difference in the availibitly between normal and infarcted hearts.
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@ Remco
What did you use to differentiate ALDH+ cells from heart to get cardiac differentiation ?
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