I have synthesized some compounds and taken 1H NMR as well as 13C NMR and in 1H NMR got two broad peaks at 15ppm and 11ppm respectively. The compounds has both OH and NH groups. I got confused which is OH peak and which is NH peak. It is possible to clarify with HMBC and HSQC NMR? if yes please can anyone help me how to interpret by using these two techniques.
what are your compound structures. Based on the chemical structures, you may be able to predict which peaks belong to the NH and OH groups from your proton spectra.
HSQC/HMBC wouldn't work for OH and would be very challenging for NH if using natural abundance. You would probably need to use tens of mM of your compound to have a chance.
If you plan on giving it a try, be sure to use mainly non-deuterated solvent, such as 90% H2O/10% D2O, since NHs and OHs exchange with the solvent.
Thanks for your answer sir. Whatever you said is obviously correct, but one of reviewer asked us like this
"Authors must check the position of OH in chromone (C2 or C4) with HMBC, HSQC experiment".
With this question I got confusion. Please help me how could I answer this question?.
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