Is it possible to have antibodies in serum (detected by ELISA) that don't neutralize influenza virus in the micro-neutralization assay?

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Is thr any simplest way to Sync or connect Android SQLite Database with Server using PHP and MySQL?

I need to connect my Android application Sqlite database with PHPMyAdmin mysql database to change the application data dynamically. I need the simplest way to do this this thing. Currently my...

08 February 2021 942 3 View

What are some good protocols for native outer membrane protein extraction from E. coli?

I am trying to extract any and all outer membrane proteins from a lab strain of e. coli (ATCC 11229) and am looking for the best general protocol to extract outer membrane proteins. All...

25 January 2021 5,736 3 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

Any instances of when an antibody will bind better if left at RT for days?

Can anyone make sense of this: an antibody was left out for 7 days on the bench at RT and binds better to a metalloprotein than if the antibody was taken out the day of the experiment?

15 April 2020 6,330 3 View

How to update inbuilt SQLite DB which is not updating itself while updating App from Play Store even after increment the database version?

how to update inbuilt SQLite DB which is not updating itself while updating App from Play Store even after increment the database version? When Database records changed in existing Database and...

05 March 2020 3,620 4 View

How do I calculate solubility of a compound using a turbidity assay?

I'm planning to measure solubility of my compound in PBS buffer by measuring the turbidity of serially diluted solutions in a microplate reader at 600 nm. Can anyone please inform me how to...

23 November 2019 3,333 3 View

Is anyone using Winepiscope 2.0 epidemiological veterinary medicine software for risk analysis?

The website version is already available but it is time consuming to feed manually. The software is in need very urgent. Please let me know ASAP.

23 October 2019 250 2 View

How to sort out a barcode mix up dilemma in a RNAseq experiment?

Hi there, So I submitted 20 RNA samples to a sequencing facility for bulk RNAseq. I got the results back and I actually suspect there might be a mix up of samples. Out of the desperation, I ran...

04 October 2019 2,801 4 View

Networking - Can you all help me network with former psychologists or counselors from corrections/juvenile detention centers, etc?

I am hoping to find connections with former psychologists and counselor who have worked with correctional populations, addictions, etc in hopes of learning from them about their experiences, any...

30 August 2019 10,008 2 View

What are some reliable methods for validating antibodies?

I want to do a pre-absorption technique, but I'm having trouble finding pure proteins of the following: ASIC 2 NFP (2F11) S46 S100P If anyone knows a good site I can purchase pure proteins or if...

16 August 2019 483 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

James Drummond

Jasmine, it is quite likely to detect antibodies in an ELISA that do not subsequently neutralize your vius. Neutralizing antibodies are a small subset of the total antibody raised against a given virus

Greg A Kirchenbaum

Absolutely! However, these antibodies may have functional activity in an ADCC assay. You will likely have to "challenge" the mice to determine if they are protected or not, as the in vitro mVN may not accurately predict protection in vivo.