How to do mono formylation of N,N-diethyl-3-methoxybenzamide?

I have attempted the formylation of the above mentioned compound using a very well reported protocol using secondary Butyl Lithium and TMEDA at - 78 degrees Celsius. I have done it quite a few...

08 February 2021 2,793 2 View

What is the minimum number of cells required for fluorescence in situ hybridization?

I have to perform a FISH experiment with limited number of cells and I am trying to estimate the minimum number of cells required to do FISH. Cells are from mammalian cell line. I failed in doing...

12 January 2021 7,887 1 View

Can you give me some Information about the generation of ROS in marine surface water?

Reactive oxygen species is a very new term i think

23 December 2020 8,416 4 View

Can someone suggest me a wear detection method for a pipe which goes under intense heat and pressure at intervals?

I am looking for a wear detection method for a pipe that goes under intense heat and pressure for short instances at intervals. this process has an average cooldown time of 8 seconds between each...

08 October 2020 3,776 2 View

Single-cell cloning from SkBr3?

Did anyone tried single-cell cloning from SkBr3 cell line. I am failing in growing cells post single cell seeding in wells. Any help is appreciated

27 September 2020 8,762 4 View

What to do when RT PCR becomes positive again after 15-20 days of 1st sample positivity?

We are getting some patients who were initially positive of RT PCR for Covid. But in retesting after 15-20 days or even 20 days after those patients remain still positive. Is it for dead RNA...

17 August 2020 9,552 9 View

How to calculate % sequence identity using Clustal W?

I am calculating % epitope conservation for different mammalian antibodies against zebrafish. I have performed the Clustal W for antigenic peptide used in raising the antibodies against the...

05 August 2020 6,399 5 View

What is the Source of Harmonic Power ?

Consider a sinusoidal supply at fundamental frequency as shown, with a series inductive reactance leading to a bus. Let a diode rectifier load be connected to the bus. The switch Sw for resistive...

24 June 2020 8,175 27 View

What is the best book on Game theory from the perspective of Computer Science and Engineering?

I do not know much about game theory. I am finding a book where different protocols and algorithms of computer science have been analyzed using the concept of game theory.

17 May 2020 4,774 7 View

How can a PhD in interdisciplinary fields can benefit a researcher in job market?

I am a PhD scholar of Humanities and my research is broadly connected to Anthropological studies and framework. I have also published with an Anthropological journal of global repute and looking...

10 May 2020 4,202 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

No title

02 March 2021 3,060 3 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View