Is it possible to get a melt with a "cumulate-like" (Hbl, Pl etc.) composition? Through either fractional crystallization or partial melting? Are there any natural or experimental examples?
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
I'm doing a Western blot and haven't seen any protein for two months. After transferring, the marker is visible, but when I detect the protein once, wash it, and try to detect another protein the...
10 June 2024 6,716 7 View
Now, I am using CRISPR/Cas 9 system to make a point mutation at THBD gene. I am wondering how can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are...
30 May 2024 9,993 0 View
Is there a convienient way to mark cancer cells that are undergoing mitosis?
05 May 2024 8,169 4 View
If the depolarization ratio of a Raman peak is less than 0.75, it is a polarized band, and if it is greater than 0.75, it is a depolarized band.
04 May 2024 2,829 0 View
I'm trying to identify some paralarvae in the central of Vietnam. I hope some experts can help me address this.
14 April 2024 6,425 0 View
I need to use burns correlation in rate based setup. But in apsen plus they do not supply burns correlation. Can i make burns correlation using fortran code in calculator block?
30 March 2024 2,647 0 View
When I did the Geotextile simulation, I set the elastic modulus, Poisson's ratio and yield stress and plastic deformation for the material as in this article, and added the fracture strain with...
14 March 2024 8,276 3 View
I want to perform a western-blotting experiment after blue-native electrophoresis, but I couldn't find a proper prestained protein ladder to indicate protein size as well as good transfer, so what...
26 February 2024 7,289 1 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,466 0 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I am new to Micromechanics and having similar problem with understanding the implementation of the formula's. I would appreciate if anyone can guide me on how to go about getting a scalar value...
30 July 2024 969 0 View
Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...
10 July 2024 5,985 0 View
I did some column tests in my lab where I needed to detect the DOC fractions using LC-OCD. For the abiotic columns I used sodium azide to restrict the biofilm formation. I did LC-OCD to detect the...
07 July 2024 468 0 View
Hi! I am working on a biophotonics project in which I need isolated but inactive CD4+ splenocytes. Every protocol and piece of literature I have found immediately activates and cultures...
02 July 2024 4,887 0 View
For Polybutylene Terephthalate (PBT) it is known that if you cool it down at fast rates to have an additional melting Peak in DSC-Scans. Androsch et al. (2015) conclude that this is due to...
30 June 2024 2,891 1 View