Is it possible to FITC the bacteria after adding to wells?

More Sadaf Dorandish's questions See All

How to delete my paper?

i want to delete my research paper.

09 July 2024 6,554 2 View

What is the limit of penetration in carbon steel for eddy current based inspection?

Theoretically, at lower A/C excitation frequencies; say around 100 Hz, penetration of 5-10 mm is achievable in carbon steel (ferromagnetic) material. But, there is literature suggesting that this...

10 March 2024 548 0 View

Could you please suggest a book related to protein engineering?

I want to gain the basic to advance knowledge about Protein Engineering.

20 January 2024 3,695 3 View

Can anyone help in modelling a case in OLGA flow assurance software?

I am modelling a case where petroleum products (gasoline, diesel) are to be handled together with Ethanol. I have created a fluid file (.mfl) with streams defined as gasoline, diesel and Ethanol...

21 November 2023 8,158 0 View

Can anyone help in modelling a fluid file in Multiflash (KBC) software?

I need to model a fluid that is a mixture of Ethanol and petroleum products (Gasoline). Which equation of state(EoS) and thermodynamic model is most suitable? Peng-Robinson, RKS or anyother?

21 November 2023 804 0 View

I have a query about carabcillin antibiotics is good option to put it in ypd agar for growing yeast or is it possible to grow them without antibiotic?

06 November 2023 342 2 View

When eddy current sensors approach carbon steel it's impedance increases unlike Al or Cu. Does this difference vanish at high frequency?

Literatures suggest that unlike non-ferromagnetic materials that lead to a decrease in impedance of the eddy current sensor, ferromagnetic materials lead to an increase in impedance of the coil....

06 July 2023 9,045 2 View

Which output parameter is better for analyzing response of eddy current sensors for metal loss (in ferromagnetic test piece)?

Some papers suggest analyzing variation in impedance of the eddy current sensor (type: PCB planar spiral sensor; absolute) while others suggest variation in frequency.

06 July 2023 1,028 0 View

Dye for FC-72?

I am unable to detect the wetting front (rate of rise) of FC-72 and I do not have a good quality IR camera. I am wandering if there is any dye which is soluble in FC-72?

11 May 2023 1,377 0 View

How to model a planar coil type Eddy current test probe in ANSYS Maxwell for determining resonant frequency?

I have modelled a planar coil in ANSYS Maxwell with Eddy Current. I am getting the B value, Resistance R, Inductance L etc. but the software seems to ignore the stray capacitance in the circuit....

26 April 2023 6,961 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

Alireza Mordadi

I've never had experience so I will glad to follow.

With my basic information I think not sterile plate can face up with problem.

In the other hand, if you add more details about your purpose it's really more helpful.

Regards.

Saba A. Hussien

Added file.

Dear Traci

If DNA fluorescent was used, when plate it's not sterile, it means maybe there is DNA, so it's not an error?

Can you explain more?

As I said I haven't any experience in this tech so I want to learn.