Is it possible to extract miRNA from plasma samples stored at - 20 for long period???

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Oscar Rafael Escate Chávez

Hello Ekhlas

I study plasma miRNAs from frozen samples. A store at -80ºC is recommended. It is possible to extract plasma miRNAs at -20ºC, high miRNAs levels could be detected by PCR but those with low levels could be unstable to detection and reproducibility in your samples.

Best regards

Ekhlas Albakri

@Oscar

Thanks for the answer

Rahul Tiwari

Yes it is possible depending upon the stability of the miRNA and its amount also. Ite recommended to store plasma at -80 degree C.

Best Regards

Unfortunately - 80 is not available in our are

Use -20 degree C at lest.