Hi Mikkel

The single cell data should be there. If you export the data in .FCS format (which is possible in BD FACSDiva) the single cell data is presented like a table where each row represents one cell and the columns represent the signal (FSC, SSC, fluorophore 1, fluorophore 2, ...). If you want to do a more sophisticated analysis (like correlating two channels for a certain subset) you can use for example R and the bioconductor package to handle your data. There are build in routines to handle the format.

https://www.bioconductor.org/

Hello Mikkel,

I was able to open a .fcs file exported from FACS Diva using JMP. This way, you will find a table for each cell and the value of each measured parameter.

Hi Mikkel

We have a free tool to convert FCS files to CSV files, which have event level values for all parameters and can be opened with standard spreadsheet applications like excel to do further analysis.

You can find it at: https://floreada.io/fcsconvert

If you are just looking for correlation between two parameters, many analysis platforms, including ours, have correlation statistics build in that you can use.

Feel free to reach out with questions