Is it possible to determine ratio of G:S (guaiacyl and syringyl) and total hydroxyl groups in lignin using HNMR using integration? At what signal (ppm) should I focused on? Attached is the NMR spectra of acetylated bamboo lignin.
Hello Syaiful, MEOD as a solvent will exchange most if not all hydroxyls to -OD and make them invisible in H-NMR. If the liginin is completely acetylated, i.e. every single hydroxyl, then it might be possible if G and S acetyle are distinguishable from the rest. The 2 ppm region does not look resolved enough to accomplish this.
Clemens
This NMR spectrum is not useful.
1. Solvent should be changed, as mentioned above by Clemens Anklin.
2. More attention should be paid to performance of the NMR measurement - especially shimming.
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