Is it possible to determine nitrogen concentration colorimetrically, using spectrophotometer in macroalgal tissue?

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Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

How can I add the metal oxide NP's to the cell lines? I have to make some drug and NP conjuhgates. How much concentration to use? Need some help?

Hi everyone. I am working on metal oxides. I need to make some conjugates of drugs and NP. But I am unsure of what concentrations to make. I have read some articles, but they haven't mentioned...

10 February 2021 3,372 2 View

Can I use a time series dependent variable in a panel regression?

Hi all Can I run a Gmm model with a time series dependent variable? I have a sample of 12 countries and years from 2000 to 2018

03 February 2021 6,491 6 View

the drug loaded nanoparticles?

Hi, I am new to this cancer cell lines field. My research is using the drug loaded nanoparticles and their effects on cancer cell lines. have a question regarding the drug loading. I have...

24 January 2021 8,258 3 View

How to calculate an endotoxin limit with a drug concentration in vp/mL?

Hello, I am trying to validate an endotoxin method for an excipient. I need to calculate my endotoxin limit. My excipient is 0.1% final product and the drug concentration is expressed in vp/mL....

20 January 2021 1,262 1 View

Why is my passive reference dye (ROX) decreasing in fluorescence as my target (FAM) increases during qPCR?

I am running qPCR on eDNA samples from rivers and have found that the vast majority of my samples show a decrease in fluorescence in my passive reference dye (ROX) while my target dye (FAM)...

15 December 2020 723 4 View

Is it possible to trap air and convert it to plasma?

How can I trap air and then convert the trapped air into plasma?

06 December 2020 624 7 View

How to deal with missing data for Reliability Analysis?

Hi, I have a dataset with missing values. I want to do a reliability analysis with SPSS and then implement the data in Mplus. Mplus can handle missing data due to the FIML. I am unsure how to...

21 November 2020 4,658 3 View

Would anyone be able to recommend some online courses/ r videos for metabolomic analysis?

Hi everyone, I would like to improve my computational skills in r, particularly in the field of metabolomics (both targeted and untargeted analysis). I think using R will make my life much...

19 November 2020 1,171 7 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View