We were trying to culture it using the Levy and Ristic method of culturing, later we used several other modified methods cited in literature (RPMI+ 40% ABS, RPMI + 50% ABS, DMEM + 40% ABS, M199 + 40% ABS). All in CO2 incubator (5% + 95% air) we were unable to culture it in a continuous manner, is it because of the low parasitemia in clinical blood samples. we used VyM buffer for washing and storing of infected sample. Can you please share some of your knowledge in this matter.
You can please go through the chapter on Bovine Babesiosis (Chapter 2.4.2.) provided by OIE. For this you can refer to the following link:-
http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.04.02_BOVINE_BABESIOSIS.pdf
Yes it is very possible, but, my concern is the technique to be used, expertise and immediacy. Because of their host specificity and obligate intra-erythrocytic nature, some researchers do advocate that the Babesia parasites are too delicate that they loose their ability to replicate when left 5 hours invitro. Therefore, in the case of low parasitaemia, there is a problem.
May i refer you to Matijatko et al., 2010 (Veterinarisky Achive)
Parasites are not microorganisms which may be easily cultured in vitro as bacteria. Culturig parasites is very difficult and I know no one which has been successfully cultured in vitro as to get full long-term development in it. So, the interest on in vitro culturing parasites always depends on the research objective you may have.
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