I want to check one plant extract, how much is it effective in case of hypertension. But I don't want to create obesity induced hypertension. Is there any other way to create hypertension model?
There are many rat models of hypertension in the world, and you do not need to create the new one. If you want a model with spontaneous hypertension, the most popular is SHR. We use the model of stress-induced hypertension, the ISIAH rats.
You can use SHR (spontaneous hypertension) rats witout any surgical intervention - as Larissa Fedoseeva stated. You can also make rats to become hypertensive (renovascular hypertension) after simple surgical procedure - eg. one kidney-one klip model.
Sodium chloride (8%) mixed with normal rat chow together with 1% in drinking water can induce hypertension after six weeks in Sprague-Dawley rats See: Obiefuna PC, Obiefuna IP., Salt-induced hypertension in rats alters the response of isolated aortic rings to cromakalim. West Indian Med J. 2001 Mar;50(1):17-21.
Dr Daniel, thanks a lot for your suggestion , i couldn't able to access this paper link, if its possible can you send me this paper ...
You can also induce hypertension by salt in the food. We made the pellest with 10% more of salt and give them for 4 weeks. The animals took 3 times more water and urinate more. When we suspended the salt, half of the animals return to normal pressure, but the other half kept hypertensive. If you suministrate the salt in the water the animals do not eat enough and are smaller.
There are multiple models depending on what type of hypertension you want to look at. In addition to the SHR there are as follows: deoxcorticostereone-acetate (DOCA)-salt, subtotal nephrectomy-salt, 2 kidney, 1 clip, 1 kidney, 1 clip as well as others.
Dr roxano , can you send me the link of that paper who has used 10% salt for 4 weeks to induce hypertension
The spontaneously hypertensive rats is classic hypertension model in rats. We induced diabetes in these rats to associate diabetes with hypertension.
https://www.researchgate.net/publication/46273239_SGLT1_protein_expression_in_plasma_membrane_of_acinar_cells_correlates_with_the_sympathetic_outflow_to_salivary_glands_in_diabetic_and_hypertensive_rats?ev=prf_pub
Article SGLT1 protein expression in plasma membrane of acinar cells ...
Another model available is the infusion of mice with angiotensin II. This can be done by the subcutaneous implantation of an osmotic pump that slowly releases angiotensin II.
Another way to induce hypertension, at least in Wistar rats, is by administrating a high salt diet for 4 weeks. If you administrate the salt in the water the rats they malnourish.
Dear Arbind
You may try the following models
1. L-name induced hypertension (doi:10.1016/j.yrtph.2012.11.001)
2. ACE estimation using FAPGG as substrate (http://www.ncbi.nlm.nih.gov/pubmed/6327119)
3. Kidney ligation method (doi:10.1016/j.lfs.2003.04.002)
I am bothered.
A scientist had commenced the usage of 4, 8 or 10% salt in diet for HTN to be induced in rodents without any reference.
why cant i start mine newly using e.g 20% as pilot study?
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