My wastewater sample is reddish brown in color and is it possible to conduct biuret test for such kind of sample? If not what kind of test would be suitable?
In principle, yes it's possible, as long as the the protein present (if any) is more than 3 amino acids long. It sounds like you will have extremely low concentrations however, so it is unlikely you will see a colour change by eye. This means changing to a spectrometer, specifically looking at 540 nm. Ideally, this would be calibrated against a known concentration beforehand.
The Biuret test may not be the most appropriate for really low concentrations, consider also the biciniconinic assay (https://en.wikipedia.org/wiki/Bicinchoninic_acid_assay) or Bradford or Lowry tests.
The reddish/brown colour is probably iron..
Good luck!
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