My wastewater sample is obtained from hydrothermal carbonization of food waste.since there is a possiblity to have amino acids in the wastewater. How to detect them?
There are several methods for amino acid analysis including OPA and AQC reagents for derivatisation and separation on C18 using water and acetonitrile solvents and fluorescence detection.
separate the solids from the supernatant to go for free amino acids, using centrifugation and filtration and go for HPLC analysis (which is very routine in most labs). Check e.g. prvious literature:
Article Anaerobic degradation of amino acids generated from the hydr...