Dear researchers I am curious to know if can we co-culture the cells of different origin. For example: muscle cells line (c2c12) and neuroblastoma (ShSY5Y) can be co-culture in the same dish?
As long as they have the same genetic background. Otherwise you may have issues with MHC I compatibility
Yes you can co-culuture cells either in the same dish or using mesh insert to keep the cells apart but still sharing the same media. But you need to make sure their genetic background is similar like Mr. Werling suggested.
Thanks you all for your answers.
Dear Ken Raj was your cells from same genetic background or different genetic background.
Hi,
The cells I use in coculture are from different genetic background. I have never encountered any problems. I am not sure why Dirk and Kasturi mentioned the need to have similar genetic background, especially since there is no immune system in the culture dish to pose any problems.
I would like to hear from Dirk and Kasturi as to the reasons for this.
Many thnaks,
Ken
Our lab works with pancreatic cancer and we have observed that when we culture a mouse stellate cells with mouse pancreatic cell, the expression pattern varies when we culture mouse stellate cells with human pancreatic cell line.
Due to different origin these two cell types have different proteomic profile that can effect your studies, especially in a co--culture experiment.
Thanks Kasturi, for your reply. I can see why you concluded that. Interesting observation. However,what you observed is due to different species. What I consider as different genetic background is different cell origins from the same species. Different species is another category of diffference altogether.
In anycase, it would be interesting if you get different protein profiles by culturing your mouse stellate cells with mouse pancreatic cells from two or more mice of different origins. However, it is an expensive thing to do and proteomic profiles tend to be rather different even when the same experiment is repeated several times. So difficult to tell.
Sorry Abhishek, for using this space to communicate with Kasturi.
Thanks
Dear Ken Raj
I think all above answer is right in their own concern.
MHC I compatibility issue will come if we deal virus infection.
Different origin issue will come if we co-culture cells from different species as @kasturi mention.
Dear Ken Raj you are also right because your culture from same species (i.e. human)
Finally thanks a lot for this great discussion.
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