If channel is potassium selective, Is it possible to block N-type inactivation with TEA in bath solution on whole cell mode conventional patch clamp. If yes can anyone suggest any reference for the same.
Perhaps my phrasing was unclear - I meant that TEA only blocks N-type inactivation when it is applied intracellularly as opposed to extracellularly, not that TEA blocks N-type inactivation and nothing else when applied intracellularly. You are correct that it blocks many K+ channels, I was assuming this would be in culture if the goal is to test a specific type of inactivation.