Hi,
I wanted to measure nitric oxide levels in human plasma, mostly using the Griess reagent. Can I use samples which have been stored in -80? Also, these plasma samples are stored in EDTA tubes. Will that affect the quality of the test?
My research objective is to find the impact of CSR on financial performance of the companies. How to measure the CSR as independent variable?
14 July 2024 2,846 5 View
I purified a protein containing His tag via Ni-NTA chromatography wherein the protein was eluted in elution buffer containing 500mM imidazole, 50 mM Tris, 500mM Nacl. Since my next step of...
31 March 2024 1,300 2 View
which journal is best for a pathology/gasteroenterology case report to be published with no publication fee?
05 March 2024 7,427 2 View
In Indian context i am trying to verify the financial performance in relation to CG and CSR. Hence for empirical analysis this is required.
19 January 2024 5,593 6 View
I want to know whether people enjoy listening to music generated through AI models and machine learning and whether people prefer this? Is there a difference between how legal professionals,...
04 December 2023 9,198 1 View
how can i add all my articles and journals in research gate including those published in research gate?
22 November 2023 5,926 3 View
I need clarity regarding the following parameters 1) Condenser Temperature ( Should I go for -50 degrees or -80 degrees) 2) Vacuum pressure to be applied
11 August 2023 8,762 1 View
Hi, I am looking to study transcriptional burst and RNA Pol II pausing at the promoter of a gene. What are the best methods to study such events at a locus? Most of the papers I saw focus on high...
04 June 2023 9,289 0 View
In order to assess the telomere length in human blood samples by qpcr, what is the best standard reference one can use for drawing the standard curve? I came across papers which use a pool of...
03 March 2023 2,324 7 View
I am inducing ischemia by OGD in vitro. If I want to estimate mitochondrial membrane potential or look at ROS levels by means of fluorescent dyes, can I fix the cells and then stain for these? I...
17 January 2023 2,257 4 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Is this circle one of the rings?
03 August 2024 5,988 0 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...
02 August 2024 7,842 0 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View