Hi,
I wanted to measure nitric oxide levels in human plasma, mostly using the Griess reagent. Can I use samples which have been stored in -80? Also, these plasma samples are stored in EDTA tubes. Will that affect the quality of the test?
I am working with rectal swab samples in the field of colorectal cancer research. Does anyone have experience (or can point towards any relevant literature) of specifically working with proteins...
02 February 2021 3,622 3 View
I am running VASP calculations involving transition metal atoms in which there are multiple unpaired electrons in the d-orbitals but I am not sure how to check the individual d-orbitals (ex:...
24 August 2020 1,947 2 View
I am doing VASP calculations with a bulk triclinic lattice system involving transition metals Cr and V. I know that you can check the spin for the entire system with the OSZICAR file from VASP but...
16 July 2020 9,052 3 View
I have 2 groups and have 7 variables. I would like to make a stacked scatter graph to present it all in one graph. Is it possible and how?
13 July 2020 2,145 3 View
Hi all, I am doing calcium imaging experiments in N2a cells and wanted to calculate the absolute calcium concentrations. Can someone help me out in figuring out how to calculate the fluorescence...
24 March 2020 7,974 1 View
Does anyone know how to derive the work function from the LOCPOT file from vasp?
24 March 2020 851 5 View
The 2 groups are parents and their children. The data is not normal. Would like the comparison without the effects of age.
21 February 2020 7,714 4 View
I have done a spearman correlation as the data is not normally distributed and now would want heritability index...am I correct in looking at heritability with this data? If yes, how do I go about?
14 February 2020 9,160 3 View
Hello Everyone, We want to measure the C-H (homolytic) bond dissociation energy of some organic compounds. Any practically feasible tool in the common analytical laboratory that can give us the...
13 February 2020 7,588 2 View
I want to purchase well characterized MDR strains of a Gram-negative bacterium from ATCC to look at modes of resistance. I am also going through literature to see if people have purchased a...
05 June 2019 7,721 1 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.
24 February 2021 1,633 4 View
I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...
24 February 2021 8,582 1 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...
23 February 2021 3,262 5 View
My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...
21 February 2021 5,656 3 View
I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...
19 February 2021 828 6 View
I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...
17 February 2021 7,402 4 View
Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...
17 February 2021 5,399 1 View