Is it possible that a gene with a tn5 insertion is still expressed ?

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Michael J. Benedik

It is possible and similar things have been observed before. Presumably in the folded protein the two catalytic domains are independent of each other and the EAL activity is encoded by the distal segment of the gene. The Tn5 insertion probably disrupts the c-terminal domain and would be in the downstream segment of the gene.

Cora Guennoc

Thank you Michael for your answer !

You are absolutely right, the EAL domain is in the c-term extremity !

Do you have some examples of publications about similar observations ?

Cora

HI Cora, my memory of those publications is vague and from about 20 years ago, so just not able to pull up any examples now. But if some come to mind I'll let you know.