Is it possible or applicable if I use the DNA extraction kit (for blood/tissue/cultured cell) to extract the DNA from helminth eggs?

More Debbra Marcel's questions See All

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View

Recommendations for Fluorescent Western Blotting?

Hi everyone, we're planning to do some protein quantification using western blot. Since a lot of our proteins of interest are of similar size, I would either have to strip and reblot a lot or...

04 November 2020 5,033 2 View

What outcomes can be expected when a leader creates a positive informal environment?

I am looking for outcomes (individual or organizational) related to the creation of a positive informal work environment. Can anybody recommend me articles on the topic? Thank you in advance....

01 August 2020 7,142 3 View

How to correct typos in RG refs?

how can one modify / correct an (annoying) typo in Research Gate mention of some publication? sometimes the name, sometimes the first name, sometimes the reference, ... is incorrect; what can one do ?

07 January 2020 9,089 5 View

How to simulate an imbalance of a rotating shaft with pointmass in abaqus?

Hi everyone, I am working on a model of a rotor to examine the deviation and critical speeds due to high speed rotation. Therefore I want to integrate an eccentricity of the shaft by creating a...

23 July 2019 4,726 17 View

How can you do statistics with normalized data?

Just briefly: I have WB quantifications from 4 different blots (4 biological repilcates) and I normalized my signal intensity values to the untreated ctrl sample. Therefore, for all 4 replicates...

14 July 2019 3,258 3 View

Internal normalization in Western Blot quantification and statistics. How to?

This might seem like a rather simple problem, but I am actually unable to find a helpful solution that specifically refers to this problem. I did several Western Blots of biological replicates...

10 July 2019 6,476 5 View

What is the significance of the graph in anova two way?

We finalized some results using ANOVA Two Way and now facing difficulties to report the statistical significance in the figures. We do not know if we use the factor significance (Factor 1, Factor...

28 October 2018 3,750 2 View

Is there a list of chemicals which lead to a higher food consumption in animals?

I am looking for some sort of list with information about how (and which) chemicals increase the amount of consumed food of animals.

12 April 2017 5,646 3 View

Boundary Conditions for Porous Contact among Two Surfaces?

I'm currently doing simulations related with contact against porous materials (Tissues) in ABAQUS. As long as I use Pore Pressure=0 as Boundary Condition everything works fine and I'm able to...

30 May 2016 3,561 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Dmitry Andreychuk

Debbra, you can try extract helminth eggs DNA with tissue DNA extraction kit, but you should warm up samples with lysis buffer (at the stage of lysis) to 60-65 С (10-15 min).

Ali Kareem

Hi,

You can try it because as you mentioned if it extracted DNA from blood snd tissues so it is fine for other organisms. Good luck and happy new year

Ali