I have PFGE typed alot of E. faecalis strains. They have obvious different patterns. I have furthermore typed them with MLST.
Several of the different PFGE patterns share the same MLST type. Is that common?
Yes, this is not surprising. By PFGE and MLST you detect complete different things. For typing, I suggest MLST or NGS
Yes, I would like to add that with MLST you are typing a limited number of loci, which apparently all your strains share. On the other hand, with the PFGE you are visualizing differences in other loci (not included in the MLST).
Dear Steffen Jørgensen,
It is possible. PFGE is more discriminatory than MLST: PFGE can detect chromosomal rearrangements. In contrast, MLST is more appropriate for strain phylogeny and large-scale epidemiology. Indeed, determination of phylogenetic relationships among strains with distinct PFGE patterns may be imprecise. For example, strains belonging to the same ST did not always cluster in a single branch based on cluster analysis of PFGE patterns. According by Johnson et al (2007), PFGE detects changes in the molecular clock better than MLST does. PFGE and MLST may be considered complementary methods, which are appropriate for studies at distinct scales, i.e. local epidemiology versus global population structure, respectively.
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