Usually the quenching happens under the FRET phenomenon, which relies in the distance of the fluorophore and the quencher. I was wondering if I set the source light to the one of the BHQ absorbance, it would absorb light.
The black hole quencher can reduce the light output from an LED by simple absorption of the light if the wavelength spectrum of light emitted by the LED overlaps the absorbance spectrum of the BHQ. Quenching would require physical contact between the BHQ and the atoms emitting the light. FRET would require that the BHQ molecules be no more than about 100 Angstroms away from the atoms.
if you pass the LED light through a solution of BHQ solution, I think some of the LED light with overlapping wavelength will be absorbed, % will depend on the intensity of your LED light. FRET is on molecular level. Could you describe in detail about your intention?
Can reduce light upon absorption or scattering effect if the solution of high concentration and non disolved particles are involved
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