Samaria Nevarez Diaz

5 Questions 2 Answers 0 Followers

Questions related from Samaria Nevarez Diaz

When a aptamer binds to protein, does the aptamer-protein molecular weight is now the addition of each molecular weight?

Im trying to figure out if when an aptamer binds to its target molecule, does the aptamer-targetmolecule has a total of the addition of each molecular weight? Lets say I have an aptamer = 12kDa...

15 October 2020 1,254 2 View

Why do I get multiple peaks of my protein when measuring molecular weight using MALDI-TOF?

I have gotten the MALDI-TOF results of my protein (Gliadin from wheat) however it shows different peaks. What does it say about my protein? (see readings attached) I need to know the total...

28 July 2020 1,300 1 View

How can I determine the accurate amount of dye to bind to protein?

I have BHQ-COOH and I would like to bind it to a certain protein. I want to calculate the exact amount of BHQ I would need to add to the protein so I dont have to filtrate every time. I cannot do...

29 December 2019 6,127 1 View

Can I obtain absorbance value (at specific wavelength) of a protein using a spectrophotometer?

I have a protein (gliadin) which I dont have the extinction coefficient. I was wondering if I can obtain the absorbance at a certain wavelength using the spectrophotometer, then calculating the...

14 July 2019 3,646 5 View

Is it possible for a quencher (E.g. BHQ) to quench LED light?

Usually the quenching happens under the FRET phenomenon, which relies in the distance of the fluorophore and the quencher. I was wondering if I set the source light to the one of the BHQ...

09 July 2019 6,763 3 View