28 September 2023 4 10K Report

Hi everyone,

I am currently studying differences in the expression of some proteins after treating cells with Western blot. My conditions are Control vs Treated, and I have repeated the Western blot 3 or 4 times. How do I perform the statistical analysis of the band density quantification? Initially, I thought that performing a Mann-Whitney U test was more appropriate since the number of repetitions is low, but I have read that it is common to use t-test. Which one is preferred and why?

Thank you.

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