Is it OK to check primer specificity using a nucleotide redundant database?

04 April 2014 1 7K Report

I want to design RT PCR primer using primer-blast (ncbi). In the organism I have added Cajanus cajan and in database Refseq mRNA and RNA. It replies that the database for this organism is not available. I have tried it with nucleotide redundant but it gives me primers so my doubt is whether it is fine to check primer specificity using a nucleotide redundant database?

Bhupendra singh Punwar

since nucleotide redudant database contain Cajanus Cajan genome sequence, you can use it

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Science method

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