Vishal R. Patil

4 Questions 16 Answers 0 Followers

Questions related from Vishal R. Patil

Is it OK to check primer specificity using a nucleotide redundant database?

I want to design RT PCR primer using primer-blast (ncbi). In the organism I have added Cajanus cajan and in database Refseq mRNA and RNA. It replies that the database for this organism is not...

04 April 2014 7,176 1 View

What are the default parameters in Primer 3 for designing real time sybr green assay primers?

see above

04 April 2014 6,822 0 View

What should I do when the DNA is viscous after resuspending and unable to settle in agarose gel? (it is polysaccharides contamination)

I am isolating genomic dna from palmyra palm (Borasus flabileffer) older leaves using CTAB method. But when I resuspend the DNA pellets in the buffer it shows a viscous jelly like appearance and...

11 November 2013 7,514 5 View

Does anyone have experience with fusarium solani showing instead of fusarium udum when isolating fungus from wilted seedling from field on PDA medium? Where could f. solani come from?

The culture shows salmon orange colour as it gives f. udum, but the identification shows fusarium solani and I was expecting f. udum. Morever, f. solani is not causing any disease on pigeonpea....

06 June 2013 6,253 1 View