I want to get a stock of my DNA of interest, but I only have transformed M15 cells and not DH5alpha cells. Is it ok if I do miniprep from an expression cell line like E. coli M15 culture and not from DH5alpha? I heard that it may be ok, but I just want to make sure.
Yes you can. But you should be aware that the M15 strain is endoA+, which means it makes a periplasmic endonuclease. Most molbio strains lack that endonuclease. This means that your DNA prep may have some nuclease contamination. It isn't a problem if you are just needing DNA to retransform, but might be a problem if you intend to do restriction digests or sequencing, depending upon how clean your miniprep is.
Thanks to all
To Michael.. Actually I need to do digestion and sequencing later. What should I do? How can I ensure to get a clean miniprep? I only have those cells containing my plasmid.
If you use a good kit to prepare your miniprep and follow the protocol for EndA+ strains, then you should be ok for digestion and sequencing. Simple "dirty" minipreps are more problematic but for doing the restriction digestions I've found that if you leave the RNA intact (do not treat with RNase) then the RNA serves as a nuclease inhibitor and your digests will be ok.
The simplest thing however is to do a quick miniprep and then retransform into DH5alpha or some similar strain that will give you high quality DNA.
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