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I am dialysing a 40KDa protein in a 3Kda dialysis membrane. The buffer that my protein is in has some glycerol remaining. So I want to make sure the glycerol is not there anymore. I ran some...
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I need to find intra-molecular interactions within a protein like hydrophobic, electrostatic, and van der Waals interactions. Do you know a program that can find this interactions and how to do...
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I want to change a Cys for an Ala in my plasmid and I want to use a kit, there are many companies that offer this type of kits, which kit would you recommend me? I have never done this before so I...
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I work with a protein that is somewhat hydrophobic for which I use denaturing conditions to purify. After purification I refold it and dialyse it, but after the dialysis my protein precipitates a...
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Is it the triple negative one (TNBC)? I will be working with MDA-MB-231 breast cancer cells, a TNBC cells line.
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I want to get a stock of my DNA of interest, but I only have transformed M15 cells and not DH5alpha cells. Is it ok if I do miniprep from an expression cell line like E. coli M15 culture and not...
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I expressed a cystein protease in M15 cells the vector is pQE30-xa. After lysis (8M urea in a tris buffer pH 8), the protein is in the pellet. I have tried many different things like 6M...
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