Marisol Romero

5 Questions 7 Answers 0 Followers

Questions related from Marisol Romero

Can glycerol pass through a 3KDa dialysis membrane?

I am dialysing a 40KDa protein in a 3Kda dialysis membrane. The buffer that my protein is in has some glycerol remaining. So I want to make sure the glycerol is not there anymore. I ran some...

28 April 2016 521 3 View

How to find intramolecular interactions within a protein?

I need to find intra-molecular interactions within a protein like hydrophobic, electrostatic, and van der Waals interactions. Do you know a program that can find this interactions and how to do...

19 February 2015 6,685 8 View

Any suggestions on how to keep my protein soluble without using too much urea?

I work with a protein that is somewhat hydrophobic for which I use denaturing conditions to purify. After purification I refold it and dialyse it, but after the dialysis my protein precipitates a...

27 September 2013 569 10 View

What is the most aggressive type of breast cancer?

Is it the triple negative one (TNBC)? I will be working with MDA-MB-231 breast cancer cells, a TNBC cells line.

18 May 2013 6,422 6 View

Is it ok if I do miniprep of my DNA of interest from an expression cell line like E. coli M15 culture?

I want to get a stock of my DNA of interest, but I only have transformed M15 cells and not DH5alpha cells. Is it ok if I do miniprep from an expression cell line like E. coli M15 culture and not...

03 December 2012 4,942 6 View