I prepared a glucose solution (4 g glucose in 20 ml water) for making bacteria competent cells and kept it in fridge but it is solidified. I have never faced such a situation. Is it a normal phenomena?
This is really an amazing case, please repeat your experiment and recheck again! . Wish you luck.
Solubility of glucose in water is temperature dependent, at higher temp you can dissolve more glucose; by heating you can get up to 40% glucose concentration But if you cool this solution, glucose will start crystallizing.
If your solution was sealed (and water did not evaporate), you can warm this solution prior to using it without any ill-effects.
It isn’t clear from your statement but I assume you are adding 4gm glucose to water and making up the volume of aqueous solution to 20 ml to get 20% concentration.
Best,
Alam
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