What is the suitable date of FITC-DMSO solution using?

Hello I want to know if I make a FITC-DMSO solution, how long time I can use it? Can I use the solution after a week? thanks for your help

08 January 2021 1,506 3 View

Why nan loss values are resulted through deep learning in python?

hello every one i want to practice deep learning as descibed for a home price prediction problem i use code as following # IMPORTING PACKAGES import pandas as pd # data processing import numpy...

07 January 2021 8,761 1 View

How can I find decay time from time resolved fluorescence curve?

Hello I put a sample in the Time Resolved Flourescence. Then the instrument gave me a time-intensity curve. Now I want to find the decay time of the sample. If you know anything about these,...

03 November 2020 1,012 4 View

How can i reduce the vegatation effect in sattelite images for mineral exploration tasks?

hello everyone, i want to examine the mineral distribution for exploration purposes using sattelite images (ETM, Aster, hyperion etc.) but how can i reduce the vegetation effect on my pocessing...

23 August 2020 716 4 View

Problem in opening VMD on Ubuntu after successfully installing?

I have installed the ubuntu and Gromacs. I also installed the VMD. It says VMd installation is complete. Enjoy! But I cannot open the VMD window! When I try to open vmd, I get all these notes but...

20 March 2020 9,762 4 View

IEEE-10 Generator 39 Bus System( simulink in matlab)?

Hi. I need IEEE-10 Generator 39 Bus System( Simulink in MATLAB). If you have it please share it with me.

14 March 2020 2,989 2 View

How to detect ethyl acetate and acetaldehyde in ethanol solution?

Hello everyone, does any one know any method to detect the existence of ethyl acetate and acetaldehyde in an ethanol medium? I have currently taken UV-VIS is there any method to detect the...

02 November 2019 1,665 3 View

I need a trick to remove DNA contamination from extracted RNA samples?

Hi, everyone I am extracting a total RNA from rat brain samples for real-time PCR studies, but unfortunately, there is DNA contamination in gel electrophoresis. I tried to remove it and I failed....

05 October 2019 897 6 View

Cancer cells and gene expression databases?

Hello, I'm trying to find the specific gene expression in various types of cancers and cancer cell lines, Is there any database in this regard?

25 September 2019 6,535 10 View

The reason why rats die in targeting both substantia nigra in LPS model of parkinson's disease?

24 July 2018 7,976 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

How do you prepare a porous crosslinked Polyvinyl alcohol and Glutaraldehyde membrane or film?

I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption

08 February 2021 7,493 3 View

Are there any fast-acting, long-term fixatives compatible with FISH?

I'm looking for a fixative that works quickly but you can safely store cells in for a longer period (i.e. a few hours). Most importantly it must be able to fix chromatin well and be compatible...

03 February 2021 7,659 7 View

How can I set up UV irradiation for crosslinking lab?

Hi, I am using a mix of 2 polymers and want to try and crosslink them. I have a UVA lamp of 365nm and have tried the lab previously at 30cm above. How would you recommend I approach the lab and...

31 January 2021 8,711 3 View

How to immobolise streptavidin to polyacrylamide using EDC/NHS?

Hi! We are aiming to bind streptavidin to polyacrylamide surface. The EDC/NHS method bounced on me as a common method, utilizing the -NH2 of polyacrylamide and -COOH of streptavidin. Is there any...

17 December 2020 7,944 2 View

How can i figure out whether nanoparticles in my synthesis crosslinked or not?

i prepare protein nanoparticles. in one of my synthesis i use trifle amount of crosslinker and the nanoparticles stick in the bottom of plate. how can i understand that nanoparticles become...

03 December 2020 559 8 View

How can my material HMW Hyaluronic acid form a gel within seconds with Eosin Y photoinitiator?

I'm currently working on developing a bioink with HMW HA-TA (hyaluronic acid/ tyramine) and Eosin Y alone. Final concentration of Polymer solution is 2.0 (w/v)% (in PBS) and Eosin Y (in DMSO)...

29 October 2020 8,771 2 View

Can someone help me to how to optimize the chromatin digestion for my CHIP experiment?

Hi everybody, I am doing CHIP experiment. The problem is that in my gel agarose I see alot of DNA with big size (longer that 1000 bp) which means there is alot of unfragmented DNA in my gel. I...

06 September 2020 1,507 5 View

Question on detection of DNA Protein covalent complexes by dot blot. How to achieve reproducubility?

I am trying to detect DNA-protein covalent crosslinks which are induced by my drug treatment. I am following the RADAR assay protocol. Briefly, I am lysing cells using a buffer containing 6M GITC,...

30 July 2020 949 4 View