What is the suitable date of FITC-DMSO solution using?

Hello I want to know if I make a FITC-DMSO solution, how long time I can use it? Can I use the solution after a week? thanks for your help

08 January 2021 1,506 3 View

Why nan loss values are resulted through deep learning in python?

hello every one i want to practice deep learning as descibed for a home price prediction problem i use code as following # IMPORTING PACKAGES import pandas as pd # data processing import numpy...

07 January 2021 8,761 1 View

How can I find decay time from time resolved fluorescence curve?

Hello I put a sample in the Time Resolved Flourescence. Then the instrument gave me a time-intensity curve. Now I want to find the decay time of the sample. If you know anything about these,...

03 November 2020 1,012 4 View

How can i reduce the vegatation effect in sattelite images for mineral exploration tasks?

hello everyone, i want to examine the mineral distribution for exploration purposes using sattelite images (ETM, Aster, hyperion etc.) but how can i reduce the vegetation effect on my pocessing...

23 August 2020 716 4 View

IEEE-10 Generator 39 Bus System( simulink in matlab)?

Hi. I need IEEE-10 Generator 39 Bus System( Simulink in MATLAB). If you have it please share it with me.

14 March 2020 2,989 2 View

I need a trick to remove DNA contamination from extracted RNA samples?

Hi, everyone I am extracting a total RNA from rat brain samples for real-time PCR studies, but unfortunately, there is DNA contamination in gel electrophoresis. I tried to remove it and I failed....

05 October 2019 897 6 View

Cancer cells and gene expression databases?

Hello, I'm trying to find the specific gene expression in various types of cancers and cancer cell lines, Is there any database in this regard?

25 September 2019 6,535 10 View

The reason why rats die in targeting both substantia nigra in LPS model of parkinson's disease?

24 July 2018 7,976 3 View

Does anyone have a finite difference Matlab code for nonlinear one dimensional ground response analysis ?

for multi layered soil - as it has been explained in Kramer's book

24 April 2018 5,081 3 View

Sewing PCR problem, any advice?

04 December 2016 7,808 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

2D wavelet transform?

Dear all I have a 2-dimensional mode shape of one concrete beam and I put the data of mode shape in a matrix (69*3) which the first column in the matrix is x coordination of my beam and the second...

10 February 2021 3,301 3 View

How can I do antigen retrieval for proliferative markers (MCM2, Ki67 etc) While preserving a sensitive endogenous reporter?

I am trying to do antigen retrieval on mouse brain sections to stain for proliferative markers like MCM2, Ki67, PCNA etc on tissue that has mCherry conjugated to a transcription factor of interest...

18 January 2021 1,023 3 View

What are technical problems working with HCT116 colon cancer cell line ? how is it maintained and cultured to study the anticancer effects of a drug?

Technical problems working with HCT116 compared with other cell line Caco-2 and HT29

07 January 2021 2,067 3 View

Is it possible to calculate the sample size of a clinical trial with the Cohen formula when there is no similar article?

We are trying to design a clinical trial on type 2 diabetes patients. The main data that we want to assess include FBS, 2hpp, HbA1c, insulin, and HOMA-IR. Also, we will assess the lipid profile...

21 December 2020 2,454 3 View

How do you confirm your beta receptor antagonist is WORKING before you implement it in a big experiment?

I want to use a beta blocker in vivo but first I want to ensure the drug is working and for how long the effects last after the drug is withdrawn. I'm thinking I can administer the drug and then...

06 December 2020 5,179 1 View

Why would looking at the expression of mecp2 in the prefrontal cortex in rett syndrome be useful?

I don't believe there's much research in this area in humans. There are mice and primate studies

05 December 2020 4,556 2 View

Kinetic modelling of oxidative dehydrogenation of ethane ?

According to below articles about kinetic modelling of ODH reactions by G. Che-Galicia and et al. there are some problems need your guidance and advices: Kinetic and reactor performance of a...

27 November 2020 9,035 3 View

Interpretation of HSV 1 and 2 Immunoflourescence ?

Hi I am M.Phil researcher in a Pathology Lab. We have purchased the SimFlour HSV 1and 2 IF kit from a reputable company. We applied it on cervical smears and cell blocks prepared from SurePath...

06 November 2020 530 1 View

What are your experience with 96-Transwell formats?

I am planning to use a 96-well format for an experiment as this will greatly improve throughput and expedite treatments. I will be using Caco-2 cells cultured apically. I need them to form...

02 November 2020 5,287 3 View