What is the suitable date of FITC-DMSO solution using?

Hello I want to know if I make a FITC-DMSO solution, how long time I can use it? Can I use the solution after a week? thanks for your help

08 January 2021 1,506 3 View

Why nan loss values are resulted through deep learning in python?

hello every one i want to practice deep learning as descibed for a home price prediction problem i use code as following # IMPORTING PACKAGES import pandas as pd # data processing import numpy...

07 January 2021 8,761 1 View

How can I find decay time from time resolved fluorescence curve?

Hello I put a sample in the Time Resolved Flourescence. Then the instrument gave me a time-intensity curve. Now I want to find the decay time of the sample. If you know anything about these,...

03 November 2020 1,012 4 View

How can i reduce the vegatation effect in sattelite images for mineral exploration tasks?

hello everyone, i want to examine the mineral distribution for exploration purposes using sattelite images (ETM, Aster, hyperion etc.) but how can i reduce the vegetation effect on my pocessing...

23 August 2020 716 4 View

IEEE-10 Generator 39 Bus System( simulink in matlab)?

Hi. I need IEEE-10 Generator 39 Bus System( Simulink in MATLAB). If you have it please share it with me.

14 March 2020 2,989 2 View

I need a trick to remove DNA contamination from extracted RNA samples?

Hi, everyone I am extracting a total RNA from rat brain samples for real-time PCR studies, but unfortunately, there is DNA contamination in gel electrophoresis. I tried to remove it and I failed....

05 October 2019 897 6 View

Cancer cells and gene expression databases?

Hello, I'm trying to find the specific gene expression in various types of cancers and cancer cell lines, Is there any database in this regard?

25 September 2019 6,535 10 View

The reason why rats die in targeting both substantia nigra in LPS model of parkinson's disease?

24 July 2018 7,976 3 View

Does anyone have a finite difference Matlab code for nonlinear one dimensional ground response analysis ?

for multi layered soil - as it has been explained in Kramer's book

24 April 2018 5,081 3 View

Cooperation invitation to establish a website?

Hi, A group of engineers are establishing a website that offers video tutorials for different engineering software including but not limited to Abaqus, Ansys, Catia and so on. They already have a...

22 December 2017 8,754 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View