Yes you can use muller hinton agar in antobiotic sensitivity test ,its the best agar.

The main media for antibiogram is MHA.

Staphylococcus and Enterococcus are able to grow on MHA and there is no need any supplements, I am sure about that but I have doubt about streptococcus so refer to CLSI.

regards

Mueller Hinton Agar developed by Muller and Hinton in 1941 is commonly used for the routine susceptibility testing of Gram negative as well as Gram positive organism by the Kirby-Bauer disk diffusion technique. We have used this medium to test susceptibility of Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, and Staphylococcus aureus.

Prof.Dr,Mahendra Pal

In addition to the aforementioned comments, Mueller Hinton agar is most suitable for susceptibility testing of Staphylococcus aureus and Enterococcus spp as they don't have very strict nutritional requirements. For streptococci however, being fastidious, you would need a basal media that has been supplemented with blood such as Mueller Hinton with Blood agar. Thanks..

If you wish to conduct drug sensitivity test of fastidious pathogens, it is necessary to add specific nutrients like blood, amino acids, vitamins in Muller and Hinton medium.Fastidious organisms, such as Heamophilus spp, Moraxella spp, Neisseria , Streptoccus spp.require enriched media like blood agar, brain heart infusion agar, milk agar for growth.

Prof.Dr.Mahendra Pal

The best medium for antibiogram is mueller hinton agar.

C'est le milieu standard pour l'étude de la sensibilité et la résistance aux antibiotiques

Yes you can use Mueller-Hinton agar for antibiotic susceptibility testing of gram-positive bacteria.

This is the best medium for performing disc diffusion assay. It has some unique properties such as

1. It is a non-selective media where nonfastidious bacteria (both aerobe and facultative anaerobe) can grow easily.

2. It contain a polysaccharide i.e starch which absorb toxins released from bacteria, so that the toxins cannot interfere with the antibiotics and you will get a proper result.

3. It exhibits batch to batch reproducibility for antibiotic susceptibility testing.

4. The levels of sulfonamide, trimethoprim, and tetracycline inhibitors are maintained in MHA.

5. This particular media allows for better diffusion of antibiotics, so that you will get a perfect zone diameter.

Just for Streptococcus spp. it's necessary to use Muller Hinton agar enriched by blood...

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Mueller and Hinton developed Mueller Hinton Agar for the isolation of pathogenic Neisseria species. Nowadays, it is more commonly used for the routine susceptibility testing of non-fastidious microorganisms by the Kirby-Bauer disk diffusion technique. Five percent sheep blood and nicotinamide adenine dinucleotide may also be added when susceptibility testing is done on Streptococcus species. This type is also commonly used for susceptibility testing of Campylobacter.

https://microbiologyinfo.com/mueller-hinton-agar-mha-composition-principle-uses-and-preparation/