I have some pure mangrove endophytic fungal colonies. I want to test them against test pathogenic strains by modified disk diffusion method by cutting agar block from the fungal colony instead of antibiotic impregnated disk. For that I have grown the test strains into nutrient broth culture and want to use them directly by cotton swab. But now I am confused weather inoculums of test strains as 0.5 McFarland standard with saline water will need to be prepared according to rational Kirby Bauer test or the test strains can be directly used from the broth culture. I have tested the growth of the test strains directly from the broth culture without matching with McFarland standard and they gave dense uniform layer on nutrient agar plate. Does it essential to prepare inoculums matching with 0.5 McFarland standard in such case and if I won’t maintain the McFarland standard what will be the possible problems because making inoculums of test strains as 0.5 McFarland for each period is time consuming and greater risk of contamination in my lab facility.
Hi Md. Sohanur Rahaman
For my opinion,
I think, you don't need to test strains as 0.5 McFarland standard with saline water
Sincerely
Hello everybody, for first step, I would advise you to follow this link first: https://en.wikipedia.org/wiki/McFarland_standards
Thank you
Thank you so much Laith sir as well as Jawad sir for your valuable suggestion. I am an undergrad student and apprentice in this field. So any further suggestions and experiences will be very appreciable and greatness.
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