Hello,

I will preface this by saying that I am not familiar with DART analysis, but in general when it comes to RNA contamination (while simultaneously, DNA is your target to isolate), most would recommend to include a step to remove RNA from your DNA samples. Many protocols where DNA is isolated you would either digest RNA or prove that there is no RNA contamination (i.e. via gel electrophoresis).

If you have time to test this idea with your assay, you could always run samples that are RNA-free versus samples where you did not include an RNA removal step, and compare your results to see if there is any significant difference. A definitive answer would lie there.