Gurpreet Singh

6 Questions 7 Answers 0 Followers

Questions related from Gurpreet Singh

How can I design allele specific primers when I have twp SNPs only few base pairs apart?

Hi there, I am trying to design allele specific primers for KASP assays to validate the SNPs from GWAS results. However, few of my SNP sequences have two or more SNPs only few base pairs apart and...

08 August 2019 2,639 5 View

Is it Important to get rid of RNA contamination if the DNA is going to be used for DArT analysis?

I have DNA extracted from Cacao plant. I am wondering if I need to get rid of RNA contamination. The samples are gonna be used for DArT analysis.

01 January 2019 6,270 1 View

Is it okay If I donot remove RNAse from my DNA samples after treating it for an hour at 37 C?

I am trying to remove RNA contamination from my DNA samples. I gave RNAse treatment for DNA resuspended in 50 ul of TE buffer. After 1 hour of treatment I reprecipitated the DNA using 1/10 V of...

01 January 2019 2,615 4 View

Why am not seeing any DNA bands from my samples?

I have extracted DNA from Cacao leaf tissue using CTAB, chloroform isoamyl alcohol method. When I quantified my samples on Nandodrop they show a conc. of 300-800 (ng/ul) in 50 ul of TE buffer. The...

01 January 2019 9,699 15 View

Can I do GWAS if I have a population of 6 crosses with 800 progeny each?

My aim is to study disease resistance on cocoa. I have a population of 6 crosses (each cross is having 800 progeny) involving a particular clone crossed with different clone in each cross.

11 November 2018 6,526 3 View

How long can I preserve plant leaves in 96% Ethanol for DNA extraction?

I will be collecting cacao leaves and thinking of preserving the leaf samples in 96% ethanol for 3-4 weeks. I am concerned it will cause any degradation of the DNA. Do I need to maintain the...

11 November 2018 5,306 6 View