I am culturing gastric cancer cell with 10% FBS. From last few months i have noticed some black dots floating in the plated media and slowly the number of dots getting increased. After reviewing some article i got a paper which suggests, these black dots are achromobacter which can be eradicated by 10mg/L ciprofloxacin and piperacillin in combination. but still i have question about PSA which we normally use as disinfectant. when we add 0.1% PSA along with 10mg/L Ciprofloxacin and Piperacillin, is it good for cellular health?
Dear Kunal,
The best approach is to use sterile techniques and to prevent any contamination. Using ciprofloxacin and piperacillin might interfere with your experimental results.
Hoping this will be helpful,
Rafik
Dear Rafik Karaman
Thanks for your valuable suggestion. But, we are using all kind of sterile techniques such as we use double distilled water (Mili-Q water) which is autoclaved along with all bottles and media filtration units, which is to be used for media preparation. we add PSA before media filtration. we revived new vials of stocked cell lines. still we get black dots in cultured media. Please suggest the solution if any.
Hello
I agree with Rafik Karaman . add we usually follow one of these protocols :
1-Dulbecco's modified Eagle's medium(4.5g/L glucose) with 10% fetal bovine serum and 10mg/L insulin.
OR
2-Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Good Luck
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