In humans, the HOMA IR is routinely calculated with fasting glucose and insulin values. However, is it essential to have fasting values when calculating HOMA-IR in mice? In the mouse, overnight fasting is stressful, resulting in counter-regulatory hormone secretion that can confound the results. Is it, therefore, better to calculate HOMA-IR in the fed state, rather than fasting, in mice?
Yes because it is during the fasting glucose values and insulin indicates the reality without any other food factor this is the good indicator of insulinoresistant
HOMA-IR is an indicator of insulin resistance. It has a reverse correlation with the ability of insulin to reduce glucose concentration. Feeding increases the concentration of glucose and insulin, which lead to high HOMA-IR. The type and the amount of consumed food significantly affect glucose concentration and insulin secretion. Thus, at the fed state HOMA-IR is not a real indicator insulin resistance.
You could get a natural fasting state by removing food (&bedding) near the end of 12 hour dark cycle and get non stressed fasting glucose and insulin near the end of the light cycle without even changing your light cycle in animal room. Just remove all food and bedding at end of dark (because they will eat feces) and then collect blood/plasma samples 10-12 hours into the light cycle. Let me know how your data turn out.
At the fasted state, HOMA-IR is a real indicator of insulin resistance. Thus HOMA-IR is calculated with fasting glucose and insulin values.
yes its essential otherwise you will get wrong result about all your correlations
It is possible to measure the glucose and insulin values in a standard group animals and use these values to calculate the homa index in your own groups. See link
https://www.ncbi.nlm.nih.gov/pubmed/23492513
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