I think that to be able to submit a paper (at least to a higher impact journal) you would need to show tri-lineage differentiation capability and as well as characterize the cells using flow cytometry.

I agree with Ena, however for lower IF journals differentiation capacity could be enough.  

I agree with Ena. It is always best to characterise your BMSC ahead to ensure their differentiation capability. 

Hello

I agree with researchers

If you want to raise the impact factor, you should describe the differentiation of cells

Good luck

Hello,

I agree with what everybody has said so far: it would be good to show that your BMMSCs are multipotent before you start your study.

Good luck!

I would also agree with everyone. However, it depends on your isolation procedure as to what cell type you are actually isolating. That is the reason why characterization is so important. Bone marrow contains a multitude of cell types, i.e., hematopoietic cells, HSCs, adipocytes, unipotent adipoblasts, fibroblasts, bipotent adipofibroblasts, chondroblasts, osteoblasts, BMMSC, VSELs, MAPCs, USSC, MIAMI, and BMSCs, just to name a few. Because VSELs and MAPCs have been reported as being pluripotent, rather than mesodermal - tripotent, I would suggest characterization for cell types from all three germ layer lineages, i.e.,  ectoderm, mesoderm, and endoderm, utilizing cell type-specific recombinant inductive agents. Also realize that a mixed population of adipoblasts, chondroblasts and osteoblasts in combination will give the same results as a pure population of the tripotent adipo-chondro-osteoblast cells. While it is an added series of steps, the best method is to clone from a single cell. That way you know that the resultant population you are working with is a pure population of cells and not a mixture of unipotent progenitor cells.

Take care.

Thank you everyone for the response and suggestions. I am working to induce them to become chondrocytes. Still in my early stage. i managed to get the expression for the cartilage specific marker (collagen type 2) and some sGAG content. But perhaps people will query whether or not I got the 'right' cells from BMMSC which gave me that 'false positive' result because I did not characterize them earlier.