What is the best internal reference for qRT-PCR assays of HepG2 cell line under the T3 treatment?

I want to treat the HepG2 cells with either T3 or GC1 (TR-beta1 agonist) for 24 hours. I'm not sure about the internal control for qRT-PCR, in order to accurate normalisation of gene...

20 July 2019 5,877 3 View

How can I reference a translated book that I have contributed as part of a team work in order to add to my CV publication list?

I'd like to add a translated text book which its translation has been done by me and other contributers , to my publication list of my CV. I'm not sure about how could I reference that? Shall I...

01 June 2019 1,990 6 View

How to model the Voids in a rock with zero elastic properties in Abaqus ?

Hello, I am trying to build a model that represents a rock with different minerals in Abaqus to recover the elastic properties like the image. My question is how to define the voids as a...

19 May 2019 6,362 7 View

Finite Element simulation on Abaqus ?

Hello, I am trying to simulate a triaxial test on a rock that has different materials properties. My target is to prove that by increasing the number of cells in the model, I will get more...

17 May 2019 9,772 3 View

Is there any tool or survey to investigate students perceptions on the importance of problem-solving skills, this is for students analysis?

is there any tool or survey to investigate students perceptions on the importance of problem-solving skills, this is for students analysis?

14 January 2019 1,362 3 View

Does anyone have protocol for lentiviral transduction of HepG2 cell lines?

Hi I need to generate TR-beta1 overexpressed HepG2 cell lines. I could not find any protocol for HepG2 transduction by lentiviral system.

30 December 2017 560 2 View

How can I choose the best plasmid vectors for lentivirus production that have my interest gene?

Hi I want to generate TR-beta overexpressed HepG2 stable cell line. For generating a lentiviral vector that have my interest gene insertion (TR-beta), that have puromycin or blasticidin...

26 December 2017 6,465 3 View

No title

For studying the role of these receptors in my gene of interest regulation in liver I need to have these cell lines

19 September 2017 4,056 1 View

How can I get access to matinspector software for promoter analysis?

every site that I have seen, just is tutorial. Is there matinspector online tool? Is it free?

27 August 2017 4,534 4 View

What are the ethical guidelines for cell line international transportation?

I mean are there any specific codes or regulations in order to govern cell line and genetic materials international transportation?

06 March 2015 6,271 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View