Hi. I am planning to inject 4T1 cells in BALB/c mice to test the efficacy of novel compounds that have inhibited proliferation of various human malignant cell lines in vitro. I am not sure if I should inject them SC or surgically. If possible send me a link for further reading. Thank you.
In our lab, we injected the 4T1 cells in BALB/c through mammary fat pad injection, and we observed aggressive growth of the cancer cells. The cancer cells metastasize to other organ such as lung observable as early as 4-5 weeks after injection.
It all depends how you want to study them. for example, if you want to study the tumor growth, you can inject S.C and then study the tumor size over a period of time. Also remember the number of injected cells would affect your tumor growth and metastasis dramatically.
I have seen papers that people inject cells S.C and after 3-5 days, when the tumor starts growing, then inject their AB or drug to see the effect of drug on tumor growth. Good luck
We do the injections subcutaneously (http://altogenlabs.com/xenograft-models/breast-cancer-xenograft/4t1-xenograft-model/), and achieve good growth curves. Usually orthotopic injections are necessary if you are monitoring tissue-specific effects and growth patterns. As Sina wrote, after the tumor has formed a drug or therapeutic is applied, and the consequent growth is monitored to give final results.
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