Well, I have always used RNAlater for preserving tissue samples or embryos, for isolation of RNA at a later time period or even for protein extraction at a later time period. But I recently observed in nature protocols, where they have advised to directly store the RNA in RNAlater.
I store RNA in nuclease free water for a long time in -80. If you however have been using RNA later you can use that as well.
I know people who (seemingly) successfully store tissue samples with RNA later at -80. Havent tried it myself though. Don´t see why it shouldn´t work.
That even I do. But my question is, once you have isolated the RNA from the tissues, do you store the RNA in RNAlater. I have always stored the isolated RNA in -80.
Thank you
Me too. I don't trust it to last long though. Haven't tried adding rnalater after purification..
Cheers,
Vegard
I store the tissue in RNAlater, but the RNA itself is kept in either nuclease-free water or TE buffer at -20 degrees C. I have had RNA stay good for years doing this (checked for integrity by gel electrophoresis). I have not stored RNA in the -80 freezer, as I do not know whether the freeze-thaw cycles from the colder temperature are harsher on nucleic acid. I have not noticed a significant difference between storing RNA in water or TE buffer, but I used the RNA for reverse transcription and qPCR; your mileage may vary.
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