Arwa Al

3 Questions 10 Answers 0 Followers

Questions related from Arwa Al

What is the differences between Methylation-Specific PCR and Sequencing after bisulfite treatment primers and how to design both?

I have a primer for a Methylation-Specific PCR (MSP) taken from the previous study and I want to design a primer for sequencing bisulfite-converted DNA for the same region that MSP primer bind to...

05 May 2017 5,147 6 View

What should be the differences and the range between tm of primers and annealing in RT-PCR?

If Tm of the both primer was 67C, is it 60C perfect as annealing temperature?

25 April 2017 4,514 11 View

Is it accepted to increase the gene expression of TP53 in cancer?

I've done RT-PCR for TP53 gene and the results showed an increase of the gene expression comparing to its normal match and as known TP53 is a tumor suppressor gene which should be down regulated...

24 February 2017 4,994 9 View