Subhash Narasimhan

5 Questions 2 Answers 0 Followers

Questions related from Subhash Narasimhan

Can i use Lactose instead of IPTG for M9 protein expression?

Hello all, Is it possible to use lactose instead of IPTG for M9 protein expression? Glucose is traditionally used as the carbon source for labelling proteins and we used 2% final in the media....

15 October 2020 3,713 4 View

Which protons are exchangable in a protein expression in 100% D2O minimal media?

Hello, I have purified a protein in minial media with 100%D2O, what i wish to know is if this is completely deuterated? I have sent this for mass spec analysis maldi with the matrix acidified...

19 February 2020 8,493 3 View

What is the ideal concentration for setting up crystallization expansion plates?

Hi, I am working with a protein stock concentration 9mg/ml which precipitates in the drop as soon as i mix the reservoir, although, it is not a repeated event because most of the crystals solved...

03 May 2019 4,430 2 View

Can you suggest any study to affirm binding of small peptides interaction with protein which require low concentration of the ligand?

Hello, I want to ask you all for suggestion of any assay of equipment which would require low concentration of ligand to study interaction with protein(K_on and K_off). I understand that ITC...

10 April 2019 4,414 7 View

Protein is colored upon purification?

HI, I am currently purifying a protein(64kDa) with rosetta gammi 2 expression. BUt, i notice a yellow colour in my fractions. It was previously not colored but, recently it is eluting in low...

12 April 2016 7,830 7 View