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Questions related from Subhash Narasimhan
Hello, I am looking for suggestions for labeling proteins lacking any cysteins and lysines. While, SDS mutation to add a cysteine or lysine are possible options. I am looking for suggestions...
30 November 2023 7,686 1 View
Hello all, Is it possible to use lactose instead of IPTG for M9 protein expression? Glucose is traditionally used as the carbon source for labelling proteins and we used 2% final in the media....
15 October 2020 3,737 4 View
Hello, I have purified a protein in minial media with 100%D2O, what i wish to know is if this is completely deuterated? I have sent this for mass spec analysis maldi with the matrix acidified...
19 February 2020 8,515 3 View
Does using taxol to stabilize tubulin polymerization to study microtubule-binding proteins either by EM or NMR acceptable? Does taxol influence a different organization of microtubule assembly...
03 February 2020 8,320 1 View
Hi, I am working with a protein stock concentration 9mg/ml which precipitates in the drop as soon as i mix the reservoir, although, it is not a repeated event because most of the crystals solved...
03 May 2019 4,442 2 View
Hello, I want to ask you all for suggestion of any assay of equipment which would require low concentration of ligand to study interaction with protein(K_on and K_off). I understand that ITC...
10 April 2019 4,426 7 View
HI, I am currently purifying a protein(64kDa) with rosetta gammi 2 expression. BUt, i notice a yellow colour in my fractions. It was previously not colored but, recently it is eluting in low...
12 April 2016 7,837 7 View
