Multiplication in grass root.

Arbuscular mycorrhizal fungi are known as obligate symbionts. Many attempts have been made, to es- tablish them in axenic culture(s) on a variety of media, but none have so far been a proven success. A few reports claim the growth of AMF (arbuscular mycorrhizal fungi) on defined media, but none of these withstand critical evaluation. Sophisticated dixenic methods using transformed root organ cultures have been successful, but they are difficult and expensive, and are not useful for general studies of mycorrhizae. It is nevertheless possible (in some instances easy) to obtain cultures of AMF open pot culture.

Dear Colleague Dipita

Greetings first. I think and understand that the first thing you will need is to obtain the pure mycorrhizal fungal strains of these fungi. You can implant trap cultures by taking rizospheric soil from the field (it is with the accompanying roots), and take pots of approximately 1,5 liter of capacity approximately and fill it with a mix of equal field soil volume and sterile sand in the autoclave (1:1, v / v) and sow sorghum in them, or Andropogon sp., or a legume like alfalfa and let grow in a greenhouse for 4 months. After that time, you can check the pot and isolate the species of arbuscular mycorrhizal fungi that reproduced to obtain pure strains

Thank you Sir for your kind reply. What I actually needed know is whether there is a growth media in which mycorrizae can be grown in culture.

Dipika, honestly there is no media so far where mycorrhizae can be grown.

Use sorgam or maize trap culture to grow mycorrhiza ( https://invam.wvu.edu/methods/culture-methods/trap-culture )

In fact I suggest you to go through entire website of INVAM ( https://invam.wvu.edu/home ) for better understanding.

https://invam.wvu.edu/home