I am looking for advice if staurosporine and its derivatives can hinder the growth of HPNE cells?
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).
04 August 2024 8,798 7 View
FYI Catalyst is MFI zeolite Si/Al = 140,25.40.11.5,15 I am using this catalyst to study methanol to DME conversion
25 July 2024 2,486 2 View
I am trying to write a detailed article Secondary Data Methods
20 May 2024 2,109 3 View
There are 33 items adding up to an overall value for qol score. How to present the results of two-step cluster analysis in the methodology for differentiating cases and controls group? Should any...
14 May 2024 7,717 2 View
Les say you have a data that is has more noise and now you will need to decide which data analysis technique will be more useful and how do you start data visualization.
09 May 2024 8,935 3 View
Dear friends, I am looking to develop a new project for the applications of displays using float glass. How and what process is needed to develop such flat panel displays what are the ideas and...
09 May 2024 3,233 0 View
Hlo I am trying to get kinetics of methanol to dimethyl ether kinetics on hzsm-5 si/al = 40 catalysts at 160 deg c I was unable to reach zeroth order kinetic regime. I am doubting that the...
05 March 2024 1,930 0 View
Dear ResearchGate colleagues, professors, and scientists, I'm encountering challenges with coated thin films produced using a sputtering system. Following treatment through tempering at 650...
11 February 2024 3,585 4 View
I have synthesized gold nanoparticles that show UV Vis peak at 522nm and were bright pink immediately after synthesis. Over a span of 24hrs, the Solution shows visible colour change from pink to...
07 February 2024 10,125 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View