Gamma radiations are used to make insects sterile as an ecofriendly way , will it be possible to provide same techanique at farmer,s level ?

There are several methods of insect pest control , one of which is the use of sterile insect technique(SIT) which is Govt.,s project. Will it be possible to use this technique at the farmer,s...

05 June 2018 1,065 8 View

Is the delocoration of the dye means degradation ?

There are several papers on the decoloration of the dyes using different method including enzymatic decoloration . Do you think that decoloration means degradation ? Explain....

05 June 2018 8,622 2 View

Which is the best method now availble for spent wash treatment to solve pollution problem ?

The efflent coming out of the sugar industries & distlllaries is a brown acidic liquid having highly acidic PH, high BOD & COD. Which is the best method so far availble for this spent...

05 June 2018 3,222 4 View

Ionic liquids are viscus in nature , how to purify ?

We are working on the organic synthesis using ionic liquids . Lets know how to purify them under such conditions ?

05 June 2018 7,285 8 View

Neem oil is used as a biopesticide .Will it be possible to increase the percentage of Azadirachtin contents in it. ?

The Neem oil is used as a biopestcides. The main constituent in the Neem seed acting as a feeding deterrent & reproduction inhibitor co . Is it possible to increase the percentage of...

05 June 2018 7,728 3 View

What are good solvents for crystallization of functionalized crown ethers ?

We are working on the synthesis of functionalized crown ethers.However, we are getting sticky product. suggest good solvent for crystallization of functionalized crown ethers.

07 August 2015 5,269 6 View

Why so far we are not successful to control mosquito population ?

We are working on the synthesis of pesticides i.e. mosquito repellents. However, so far we are not able to control mosquito population effectively ?

07 August 2015 1,777 2 View

What is difference between plant DNA & animal DNA. Can we transfer plant DNA in to animal ?

09 October 2014 9,620 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View